文章基本信息

标题：Distinctive populations of CD4 +T cells associated with vaccine efficacy
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作者：Therese Woodring ; Colin N. Dewey ; Lucas Dos Santos Dias 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：9
页码：1-24
DOI：10.1016/j.isci.2022.104934
语种：English
出版社：Elsevier
摘要：SummaryMemory T cells underpin vaccine-induced immunity but are not yet fully understood. To distinguish features of memory cells that confer protective immunity, we used single cell transcriptome analysis to compare antigen-specific CD4+T cells recalled to lungs of mice that received a protective or nonprotective subunit vaccine followed by challenge with a fungal pathogen. We unexpectedly found populations specific to protection that expressed a strong type I interferon response signature, whose distinctive transcriptional signature appeared unconventionally dependent on IFN-γ receptor. We also detected a unique population enriched in protection that highly expressed the gene for the natural killer cell marker NKG7. Lastly, we detected differences in TCR gene use and in Th1- and Th17-skewed responses after protective and nonprotective vaccine, respectively, reflecting heterogeneousIfng- andIl17a-expressing populations. Our findings highlight key features of transcriptionally diverse and distinctive antigen-specific T cells associated with protective vaccine-induced immunity.Graphical abstractDisplay OmittedHighlights•Protective and nonprotective vaccines generate distinct T cells in fungal infection•A strong type I interferon signal is seen among CD4 T cells in protective immunity•Th1 bias is seen with protective immunity; Th17 bias with nonprotective immunity•Nkg7-expressing CD4 T cells are enriched in protective immunityImmunology; Immune response; Transcriptomics.