文章基本信息

标题：Extrusion Improves the Antihypertensive Potential of a Kabuli Chickpea ( Cicer arietinum L.) Protein Hydrolysate
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作者：Jeanett Chávez-Ontiveros ; Cuauhtémoc Reyes-Moreno ; Giovanni Isaí Ramírez-Torres 等
期刊名称：Foods
电子版ISSN：2304-8158
出版年度：2022
卷号：11
期号：17
页码：1-11
DOI：10.3390/foods11172562
语种：English
出版社：MDPI Publishing
摘要：Chickpea hydrolysates could have antihypertensive potential, but there are no evaluations in vivo. Thus, the antihypertensive potential of a chickpea protein hydrolysate obtained before and after extrusion (a process that modifies protein digestibility) was evaluated. Protein precipitates were obtained from extruded and unextruded chickpea flours by isoelectric precipitation and hydrolyzed (α-amylase/pepsin/pancreatin). Chemical composition was determined (standard methods). ACE-I inhibition assays were carried out using a colorimetric test. For antihypertensive effect evaluations, spontaneously hypertensive rats ( n = 8) received the treatments intragastrically (extruded or unextruded hydrolysate (1.2 g/kg), captopril (25 mg/kg), or water only). Fat, ash, and carbohydrate contents were lower in extruded chickpea flour ( p < 0.05 versus unextruded). The protein content varied between protein precipitates (91.03%/78.66% unextruded/extruded (dry basis)) ( p < 0.05). The hydrolysates’ IC50 values (mg/mL) were 0.2834 (unextruded)/0.3218 (extruded) ( p > 0.05). All treatments lowered the blood pressure ( p < 0.05 vs. water). The extruded hydrolysate showed a more potent antihypertensive effect than the unextruded one ( p < 0.05), an effect similar to captopril ( p > 0.05). The results suggest that protein extrusion can be used to generate protein hydrolysates with improved health benefits. The findings have implications for the design and production of functional foods that could help to prevent hypertension or serve as an adjunct in its treatment.
关键词：chickpea;hydrolysate;extrusion;antihypertensive;ACE-I;Kabuli;hypertension