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标题：Multiple free-radical scavenging capacity in serum
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作者：Shigeru Oowada ; Nobuyuki Endo ; Hiromi Kameya 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2012
卷号：51
期号：2
页码：117-121
DOI：10.3164/jcbn.11-113
出版社：The Society for Free Radical Research Japan
摘要：We have developed a method to determine serum scavenging-capacity profile against multiple free radical species, namely hydroxyl radical, superoxide radical, alkoxyl radical, alkylperoxyl radical, alkyl radical, and singlet oxygen. This method was applied to a cohort of chronic kidney disease patients. Each free radical species was produced with a common experimental procedure; i.e., uv/visible-light photolysis of free-radical precursor/sensitizer. The decrease in free-radical concentration by the presence of serum was quantified with electron spin resonance spin trapping method, from which the scavenging capacity was calculated. There was a significant capacity change in the disease group ( n = 45) as compared with the healthy control group ( n = 30). The percent values of disease’s scavenging capacity with respect to control group indicated statistically significant differences in all free-radical species except alkylperoxyl radical, i.e., hydroxyl radical, 73 ± 12% ( p = 0.001); superoxide radical, 158 ± 50% ( p = 0.001); alkoxyl radical, 121 ± 30% ( p = 0.005); alkylperoxyl radical, 123 ± 32% ( p >0.1); alkyl radical, 26 ± 14% ( p = 0.001); and singlet oxygen, 57 ± 18% ( p = 0.001). The scavenging capacity profile was illustrated using a radar chart, clearly demonstrating the characteristic change in the disease group. Although the cause of the scavenging capacity change by the disease state is not completely understood, the profile of multiple radical scavenging capacities may become a useful diagnostic tool.
关键词：multiple free radical;MULTIS;serum;ESR spin trapping;chronic kidney disease