文章基本信息

标题：Generation Mechanism of Radical Species by Tyrosine-Tyrosinase Reaction
本地全文：下载
作者：Mika Tada ; Masahiro Kohno ; Shigenobu Kasai 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2010
卷号：47
期号：2
页码：162-166
DOI：10.3164/jcbn.10-48
出版社：The Society for Free Radical Research Japan
摘要：Alleviated melanin formation in the skin through inhibition of tyrosine-tyrosinase reaction is one of the major targets of cosmetics for whitening ability. Since melanin has a pivotal role for photoprotection, there are pros and cons of inhibition of melanin formation. This study applying electron spin resonance (ESR)-spin trapping method revealed that ^•H and ^•OH are generated through tyrosine-tyrosinase reaction. When deuterium water was used instead of H₂O, the signal of 5,5-dimethyl-1-pyrroline N -oxide (DMPO)-H (a spin adduct of DMPO and ^•H) greatly decreased, whilst DMPO-OH (a spin adduct of DMPO and ^•OH) did not. Thus, it is suggested that ^•H was derived from H₂O, and ^•OH through oxidative catalytic process of tyrosine to dopaquinone. Our study suggests that tyrosinase inhibitors might contribute to alleviate the oxidative damage of the skin by inhibiting ^•OH generation via the enzyme reaction.
关键词：tyrosine;tyrosinase;radical species