文章基本信息

标题：Scavenging or Quenching Effect of Melanin on Superoxide Anion and Singlet Oxygen
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作者：Mika Tada ; Masahiro Kohno ; Yoshimi Niwano 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2010
卷号：46
期号：3
页码：224-228
DOI：10.3164/jcbn.09-84
出版社：The Society for Free Radical Research Japan
摘要：Although photoprotective properties of skin melanin have been well documented, a few studies on the effect of melanin on reactive oxygen species (ROS) generated by ultraviolet (UV) irradiation have been reported. To study the interaction of melanin with ROS, scavenging or quenching effect of melanin on O₂^•− and ¹O₂ was examined by electron spin resonance (ESR)-spin trapping methods and a spectrophotometric method, respectively. Melanin potently interacted with O₂^•− generated in a hypoxanthine (HPX)-xanthine oxidase (XOD) reaction, and with ¹O₂ generated from a peroxidase, H₂O₂, and halide system. In the HPX-XOD reaction, it was proved that melanin doses not interfere with the enzyme reaction. It is confirmed that one of the mechanisms by which melanin protects UV-induced skin damage is likely scavenging or quenching activity against ROS such as O₂^•− and ¹O₂.
关键词：melanin;reactive oxygen species;antioxidant