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标题：Radical Scavenging Activity of the Essential Oil of Silver Fir ( Abies alba )
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作者：Seun-Ah Yang ; Sang-Kyung Jeon ; Eun-Jung Lee 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2009
卷号：44
期号：3
页码：253-259
DOI：10.3164/jcbn.08-240
出版社：The Society for Free Radical Research Japan
摘要：The essential oil of silver fir ( Abies alba ) is known to help respiratory system and have easing and soothing effect for muscle. In the present study, we investigated the chemical composition, cytotoxicity and its biological activities of silver fir ( Abies alba ) essential oil. The composition of the oil was analyzed by GC-MS and bornyl acetate (30.31%), camphene (19.81%), 3-carene (13.85%), tricyclene (12.90%), dl-limonene (7.50%), α-pinene (2.87%), caryophyllene (2.18%), β-phellandrene (2.13%), borneol (1.74%), bicyclo[2.2.1]hept-2-ene,2,3-dimethyl (1.64%) and α-terpinene (1.24%) were the major components in the oil. The results tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay indicated that the oil showed no cytotoxic effect, at concentrations of 1 and 5%, for as long as 24 and 3 h, respectively. The antiradical capacity was evaluated by measuring the scavenging activity of the essential oil on the 2,20-diphenylpicrylhydrazyl (DPPH) and 2,2'-azino-bis 3-ethyl benzothiazoline-6-sulfonic acid (ABTS) radicals. The oil was able to reduce the both radicals dose-dependently, and the concentration required for 50% reduction (RC₅₀) against DPPH radicals (2.7 ± 0.63%) was lower than ABTS radicals (8.5 ± 0.27%). The antibacterial activity of the oil was also evaluated using disc diffusion method against Staphylococcus aureus , Streptococcus mutans , Listeria monocytogenes , Acinetobacter baumannii , Escherichia coli , and Vibrio parahaemolyticcus . The oil exhibited no antibacterial activity against all the bacterial strains tested except S. aureus of mild activity.
关键词：silver fir essential oil ; Abies alba ; GC-MS ; antiradical ; antibacterial