文章基本信息

标题：Antioxidant Activities of Uyaku (Lindera Strychnifolia) Leaf Extract: A Natural Extract Used in Traditional Medicine
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作者：Yasuko Noda ; Akitane Mori
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2007
卷号：41
期号：2
页码：139-145
DOI：10.3164/jcbn.2007019
出版社：The Society for Free Radical Research Japan
摘要：Uyaku ( Lindera strychnifolia , Sieb. et Zucc. ) is used in traditional Asian medicine to treat stomach and renal diseases, neuralgia, rheumatism, and aging. In this study, the effects of lyophilized extracts on hydroxyl (^·OH) and superoxide (O₂^·⁻) radicals were examined using an electron spin resonance (ESR) spectrometer with the spin trap, 5,5'-dimethyl-1-pyrroline-N-oxide. Inhibitory effects were assessed using the following reagents: for nitric oxide (NO^·), the Griess reagent; for (Fe²⁺ + H₂O₂)-induced lipid peroxidation, 2-thiobarbituric acid; for (Fe²⁺ + H₂O₂)-induced protein carbonyl, 2,4-dinitrophenylhydrazine. Analysis of ESR data of the extracts indicated the direct ^·OH and O₂^·⁻ scavenging. The extracts scavenged NO^· in a dose-dependent manner, inhibited lipid peroxidation of linolenic acid, and protein carbonyl formation in bovine serum albumin. In conclusion, the Uyaku leaf hot-water extract has potent scavenging activity against reactive oxygen species and reactive nitrogen species, and effectively inhibited lipid peroxidation. These results might contribute to understanding age-associated or free radical-related diseases induced by excess reactive oxygen and also nitrogen species.
关键词：Uyaku;natural antioxidant;lipid peroxidation