文章基本信息

标题：Various Molecular Species of Diacylglycerol Hydroperoxide Activate Human Neutrophils via PKC Activation
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作者：Yasuhiro Kambayashi ; Susumu Takekoshi ; Yutaka Tanino 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2007
卷号：41
期号：1
页码：68-75
DOI：10.3164/jcbn.2007009
出版社：The Society for Free Radical Research Japan
摘要：We have proposed that diacylglycerol hydroperoxide-induced unregulated signal transduction causes oxidative stress-related diseases. In this study, we investigated which molecular species of diacylglycerol hydroperoxide activated human peripheral neutrophils. All diacylglycerol hydroperoxides, diacylglycerol hydroxides, and diacyglycerols tested in the present study induced superoxide production by neutrophils. The ability to activate neutrophils among molecular species containing the same fatty acid composition was as follows; diacylglycerol hydroperoxide>diacylglycerol hydroxide≥diacylglycerol. The diacylglycerol hydroperoxide composed of linoleate was a stronger activator for neutrophils than that composed of arachidonate. 1-Palmitoyl-2-linoleoylglycerol hydroperoxide (PLG-OOH) was the strongest stimulator for neutrophils. We reconfirmed that PLG-OOH activated protein kinase C (PKC) in neutrophils. PLG-OOH induced the phosphorylation of p47^phox, a substrate of PKC and a cytosolic component of NADPH oxidase, in neutrophils, as did N- formyl-methionyl-leucyl-phenylalanine or 4β-phorbol-12β-myristate-13α-acetate. Moreover, the time course of p47^phox phosphorylation was comparable to that of superoxide production. These results suggest that PLG-OOH activated intracellular protein kinase C. PLG-OOH, produced via an uncontrolled process, can act as a biological second messenger to cause inflammatory disease from oxidative stress.
关键词：1-palmitoyl-2-linoleoylglycerol hydroperoxide;neutrophil;oxidative stress;protein kinase C;p47phox