文章基本信息

标题：Japanese Kelp (Kombu) Extract Suppressed the Formation of Aberrant Crypt Foci in Azoxymethane Challenged Mouse Colon
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作者：Swadesh K. Das ; Takashi Hashimoto ; Masaki Baba 等
期刊名称：Journal of Clinical Biochemistry and Nutrition
印刷版ISSN：0912-0009
电子版ISSN：1880-5086
出版年度：2006
卷号：38
期号：2
页码：119-125
DOI：10.3164/jcbn.38.119
出版社：The Society for Free Radical Research Japan
摘要：Japanese kelp (kombu) includes a xanthophy, fucoxanthin. In the present study, the chemopreventive activity against the formation of aberrant crypt foci (ACF, a preneoplastic marker for colon cancer) was examined. Four groups of mice were housed with drinking water containing 5 mg/ml of a kombu ethanol extract, and 0.05 mg/ml and 0.1 mg/ml of purified fucoxanthin, and with water alone as the positive control. They were subcutaneously injected with 10 mg/kg b.w. of azoxymethane (AOM) on the 1^st and 8^th days. Another group served as a negative control without kombu and AOM. The positive control produced a substantial number of ACF by the 28^th day. Significant reductions were found in the frequency of ACF: 36% for the kombu extract, 30% for 0.05 mg/ml fucoxanthin and 35% for 0.1 mg/ml fucoxanthin. In the liver of these mice, the kombu extract raised the level of activity of glutathione S-transferase (GST) and quinone reductase, and stimulated the antioxidant responsive element (ARE). Fucoxanthin enhanced the GST activity but did not affect the ARE. Thus, one of the kombu ingredients, fucoxanthin, can suppress the ACF formation induced by the colon carcinogen AOM, and other unknown ingredients can induce the ARE activation.
关键词：Japanese kelp;Fucoxanthin;ACF;colon cancer;phase II enzymes