文章基本信息

标题：LncRNA and mRNA expression profiles in brown adipose tissue of obesity-prone and obesity-resistant mice
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作者：Congcong Wang ; Meng Duan ; Jinhua Lin 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：8
页码：1-20
DOI：10.1016/j.isci.2022.104809
语种：English
出版社：Elsevier
摘要：SummaryObesity-prone or obesity-resistant phenotypes can exist in individuals who consume the same diet type. Brown adipose tissue functions to dissipate energy in response to cold exposure or overfeeding. Long noncoding RNAs play important roles in a wide range of biological processes. However, systematic examination of lncRNAs in phenotypically divergent mice has not yet been reported. Here, the lncRNA expression profiles in BAT of HFD-induced C57BL/6J mice were investigated by high-throughput RNA sequencing. Genes that play roles in thermogenesis and related pathways were identified. We found lncRNA (Gm44502) may play a thermogenic role in obesity resistance by interacting with six mRNAs. Our results also indicated that seven differentially expressed lncRNAs (4930528G23Rik, Gm39490, Gm5627, Gm15551, Gm16083, Gm36860, Gm42002) may play roles in reducing heat production in obesity susceptibility by interacting with seven differentially expressed mRNAs. The screened lncRNAs may participate in the pathogenesis of weight regulation and provide insight into obesity therapy.Graphical abstractDisplay OmittedHighlights•First lncRNA profiles in BAT of OR and OP mice via bioinformatic analysis•Gm44502 may play a thermogenic role by interacting with 6 mRNAs•7 DElncRNAs may reduce thermogenesis by interacting with 7 DEmRNAs•Validation of expression changes of candidate genes in BAT byin vivoorin vitroPhysiology; Omics; Transcriptomics