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标题：Aldh2 Knockout Mice Were More Sensitive to DNA Damage in Leukocytes due to Ethyl Tertiary Butyl Ether Exposure
本地全文：下载
作者：Zuquan WENG ; Megumi SUDA ; Katsumi OHTANI 等
期刊名称：Industrial Health
印刷版ISSN：0019-8366
电子版ISSN：1880-8026
出版年度：2011
卷号：49
期号：3
页码：396-399
DOI：10.2486/indhealth.MS1188
出版社：National Institute of Occupational Safety and Health
摘要：To clarify the genotoxicity of ethyl tertiary butyl ether (ETBE), a gasoline additive, male and female C57BL/6 mice of Aldh2 +/+ and Aldh2 –/– genotypes, aged 8 wk, were exposed to 0, 500, 1,750, or 5,000 ppm ETBE for 6 h/day, 5 d per week for 13 wk. DNA damage in leukocytes was measured by the alkaline comet assay and expressed quantitatively as Tail Intensity (TI). For male mice, TI was significantly higher in all three groups exposed to ETBE than in those without exposure within Aldh2 –/– mice, whereas within Aldh2 +/+ mice, TI increased only in those exposed to 5,000 ppm of ETBE as compared with mice without exposure. For female mice, a significant increase in TI values was observed in the group exposed to 5,000 ppm of ETBE as compared with those without exposure within Aldh2 –/– mice; TI in Aldh2 –/– mice exposed to 1,750 and 5,000 ppm was significantly higher than in Aldh2 +/+ mice without exposure. TI did not significantly increase in any of the groups exposed to ETBE within female Aldh2 +/+ mice. Based on the results we suggest that Aldh2 –/– mice are more sensitive to DNA damage caused by ETBE than Aldh2 +/+ mice and that males seem more susceptible to this effect than females.
关键词：Ethyl tertiary butyl ether;Genotoxicity;Aldh2 knockout mice;Comet assay;Leukocyte;Aldehyde dehydrogenase;Acetaldehyde