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标题：Investigation of Gene Expression of MMP-2 and TIMP-2 mRNA in Rat Lung in Inhaled Nickel Oxide and Titanium Dioxide Nanoparticles
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作者：Yasuo MORIMOTO ; Takako OYABU ; Akira OGAMI 等
期刊名称：Industrial Health
印刷版ISSN：0019-8366
电子版ISSN：1880-8026
出版年度：2011
卷号：49
期号：3
页码：344-352
DOI：10.2486/indhealth.MS1218
出版社：National Institute of Occupational Safety and Health
摘要：In order to investigate whether or not dispersed nanoparticles have an effect of inflammation and fibrosis on animals, we developed a nanoparticle generation system and examined the gene expression of matrix metalloproteinase (MMP) and tissue inhibitor matrix proteinase (TIMP) in rat lung containing inhaled nickel oxide (NiO) or titanium dioxide (TiO₂) nanoparticles. In both experiments, Wistar male rats were exposed to NiO or TiO₂ nanoparticles for 4 wk (6 h/day). The geometric mean diameters of NiO and TiO₂ in the chamber were 139 ± 12 nm and 51 ± 9 nm, respectively. The average concentration of the particle number of NiO and TiO₂ was 1.0E+05 /cm³ and 2.8E+05 /cm³, respectively. At 4 d, 1 and 3 months after the end of the inhalation, the rats exposed to these particles were sacrificed and the gene expressions of MMP-2, TIMP-2 and type I collagen were measured using RT-PCR. Pathological finding revealed that there was minimum inflammation with nickel oxide only at 4 d and no change with titanium oxide. However, there were no changes of the gene expression of MMP-2, TIMP-2, and type I collagen in either the NiO or TiO₂ exposure groups. In this study, inhalation of nickel oxide and titanium dioxide nanoparticles did not induce the gene expression of MMP-2 and TIMP-2 mRNA in rat lungs.
关键词：Nanoparticle;Inhalation study;Nickel oxide;Titanium dioxide;Matrix