文章基本信息

标题：Molecular detection of Aspergilli from commercial chicken in selected areas of Bangladesh
本地全文：下载
作者：Md. Yeasin Arafat ; Md. Monowarul Islam ; Shamim Ahamed 等
期刊名称：Journal of Advanced Veterinary and Animal Research
印刷版ISSN：2311-7710
出版年度：2022
卷号：9
期号：2
页码：184-190
DOI：10.5455/javar.2022.i583
语种：English
出版社：Bangladesh Agricultural University, Faculty of Veterinary Science
摘要：Objectives: This study was designed to isolate, identify, and determine the prevalence of Aspergilli in commercial chicken in selected areas of Bangladesh. Materials and Methods: A total of 50 lung samples from suspected dead chickens, comprising broilers ( n = 32) and layers ( n = 18), aged between 5 days and 45 weeks, were collected from poultry farms located in the Gazipur district in Bangladesh. Fungi were primarily identified based on the colony morphology using potato dextrose agar (PDA). DNA was extracted from the suspected colonies. Aspegillusspp. was detected by genus-specific ASAP-1 and ASAP-2. Aspergillusspp. were then screened by polymerase chain reaction targeting Aspergillus flavus(FLA-1 and FLA-2), Aspergillus fumigatus(ASPU and Af3r), and Aspergillus niger(ASPU and Nilr). Results: The overall prevalence of Aspergillusspp. was 44% ( n = 22/50; p <0.05). Among the Aspergilli, A. flavuswas detected in 10% ( n = 5/50) of the samples. Similarly, A. fumigatus and A. nigerwere detected at 26% ( n = 13/50) and 8% ( n = 4/50) respectively. Three samples were associated with more than one fungus; two fungi ( A. flavus and A. niger) were in two samples, and three fungi ( A. flavus, A. fumigatus, and A. niger) were in one sample. Conclusion: Isolation and prevalence of Aspergillusspp. in commercial chicken were studied for the first time in Bangladesh.
关键词：Aspergilli;A. flavus;A. fumigatus;A. niger;chicken;prevalence;PCR