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标题：Development of Nanomaterial-Modified Impedimetric Aptasensor—A Single-Step Strategy for 3,4-Methylenedioxymethylamphetamine Detection
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作者：Shringika Soni ; Utkarsh Jain ; Donald H. Burke 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2022
卷号：12
期号：7
DOI：10.3390/bios12070538
语种：English
出版社：MDPI Publishing
摘要：Abstract Developing rapid, sensitive detection methods for 3,4-Methylenedioxymethylamphetamine (MDMA) is crucial to reduce its current misuse in the world population. With that aim, we developed an aptamer-modified tin nanoparticle (SnNP)-based nanoarchitecture as an electrochemical sensor in this study. This platform exhibited a high electron transfer rate with enhanced conductivity arising from its large surface area in comparison to the bare electrode. This observation was explained by the 40-fold higher electroactive surface area of SnNPs@Au, which provided a large space for 1.0 μM Apt MDMA (0.68 ± 0.36 × 10 12 molecule/cm 2) immobilization and yielded a significant electrochemical response in the presence of MDMA. Furthermore, the Apt MDMA -modified SnNPs@Au sensing platform proved to be a simple yet ultrasensitive analytical device for MDMA detection in spiked biological and water samples. This novel electrochemical aptasensor showed good linearity in the range of 0.01–1.0 nM for MDMA (R 2 = 0.97) with a limit of detection of 0.33 nM and a sensitivity of 0.54 ohm/nM. In addition, the device showed high accuracy and stability along with signal recoveries in the range of 92–96.7% (Relative Standard Deviation, RSD, 1.1–2.18%). In conclusion, the proposed aptasensor developed here is the first to combine SnNPs and aptamers for illicit compound detection, and it offers a reliable platform for recreational drug detection.
关键词：3,4-methylenedioxymethylamphetamine;electrochemical sensor;aptamer;tin nanoparticles;recreational drugs;forensic