文章基本信息

标题：A New Biorecognition-Element-Free IDμE Sensor for the Identification and Quantification of E. coli
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作者：Yung-Kai Lin ; Hsing-Ju Wu ; Nguyen Van Hieu 等
期刊名称：Biosensors
电子版ISSN：2079-6374
出版年度：2022
卷号：12
期号：8
DOI：10.3390/bios12080561
语种：English
出版社：MDPI Publishing
摘要：The label-free biosensor has emerged as an effective tool for the purpose of early detection of causative pathogens such as Escherichia coli as a preventive measure. In this study, a biorecognition-element-free interdigitated microelectrode (IDμE) sensor is designed and developed with this in mind, with good reliability and affordability. Results show that the designed sensor can identify E. coli with good selectivity using an impedance and capacitance of 7.69 MHz. At its optimum impedance of 1.3 kHz, the IDμE sensor can reliably quantify E. coli in a range of measurement (10 3.2~10 6 cfu/mL), linearity (R 2 = 0.97), sensitivity (18.15 kΩ/log (cfu/mL)), and limit of detection (10 3.2 cfu/mL). In summary, the IDμE sensor developed possesses high potential for industrial and clinical applications.
关键词：Escherichia coli;interdigitated microelectrodes;biorecognition element free;impedance;capacitance