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标题：Ultraviolet germicidal irradiation of melted snow and ice samples: inactivation of microorganisms and effects on insoluble microparticles
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作者：Fumio Nakazawa ; Kumiko Goto-Azuma
期刊名称：Journal of Glaciology
印刷版ISSN：0022-1430
电子版ISSN：1727-5652
出版年度：2022
卷号：68
期号：267
页码：166-173
DOI：10.1017/jog.2021.78
语种：English
出版社：Cambridge University Press
摘要：The long-term refrigerated storage of melted snow and/or ice samples for analyses of insoluble microparticles (hereafter, microparticles) may be limited by increases in the biological particle concentration caused by microbial growth after ~1–2 weeks. In this study, we examined an ultraviolet (UV) disinfection method for the storage of melted snow and/or ice samples and determined the effects of this method on microparticles. Surface snow obtained from Glacier No. 31 in the Suntar-Khayata Range, eastern Siberia, Russia was divided into two portions for UV treatment and untreated controls. Microparticle concentrations and size distributions (in the range of 0.52–12.0 μm) in the samples were measured using a Coulter counter. Whereas the microparticle concentration in untreated samples increased, no obvious increase was observed over 53 d in the samples subjected to UV treatment. Microbial growth was detected in only untreated samples using a viable particle counter. In addition, the original microparticle concentrations and size distributions were unaffected by UV treatment. Our results demonstrated that the microparticle size distribution in untreated melted water samples reflects the growth, decomposition and succession of microorganisms over time and further indicate that UV irradiation is effective for long-term storage for microparticle analysis.
关键词：Biological particle;culture;glacier;snow;sterilization