文章基本信息

标题：Engineering of a Multi-Epitope Subunit Vaccine Against SASRS-CoV-2 Through the Viroinformatic Approach
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作者：Aamir Shehzada ; Christijogo Sumartono ; Jusak Nugraha 等
期刊名称：Karbala International Journal of Modern Science
印刷版ISSN：2405-609X
电子版ISSN：2405-609X
出版年度：2022
卷号：8
期号：2
页码：275-291
DOI：10.33640/2405-609X.3221
语种：English
出版社：Elsevier
摘要：The COVID-19 outbreak has infected millions of people worldwide, but no vaccine has been discovered to combat it efficiently. This research aims to design a multi-epitope vaccine using highly efficient B- and T-cell epitopes from the SARS-CoV-2 Surabaya isolate through a viroinformatic approach. First, the putative epitopes were linked together to develop tertiary structures and then docked with toll-like receptor 4 (TLR-4) that demonstrated a robust interaction with a low eigenvalue of 4.816138 e-06. Furthermore, the structure's high immunogenic response was observed and successfully cloned into the expression vector pET28a (+). This implies that the designed vaccine can prove effective in combating SARS-CoV-2.
关键词：Bioinformatics;SARS;CoV;2;public health;MHC;I and MHC;II;multi;epitope vaccine