文章基本信息

标题：Leptin gene polymorphism Rs7799039; G2548A, metabolic and oxidative stress markers in polycystic ovarian syndrome
本地全文：下载
作者：Shilpa S. Shetty ; N. Suchetha Kumari ; Pravesh Hegde 等
期刊名称：Journal of King Saud University - Science
印刷版ISSN：1018-3647
出版年度：2022
卷号：34
期号：6
页码：1-5
DOI：10.1016/j.jksus.2022.102222
语种：English
出版社：Elsevier
摘要：AbstractObjectivesStress which includes various similar categories such as emotional, inflammatory, oxidative, and metabolic stress is an important component of the Polycystic Ovarian Syndrome (PCOS). The current study examines the relationship of leptin gene polymorphism with metabolic, oxidative stress, and inflammatory markers in polycystic ovarian syndrome.MethodsA total of 300 subjects were enlisted (Control-150; PCOS-150). Commercially available kits were used to measure the glycemic, inflammatory, and lipid profile status. Standard methods were used to examine oxidative stress markers. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was carried out for SNP rs7799039 genotyping. Statistical Package for Social Sciences (SPSS) software (version 20.0, IBM, Armonk, NY, USA) was used for statistical analyses.ResultsThe groups exhibited a difference with statistical significance in terms of body mass index (BMI), insulin, HOMA-IR, Triglyceride, Follicle-stimulating hormone (FSH), Testosterone, vitamin D, leptin, TNF-alpha, IL-4, IL-10, total antioxidant capacity, and myeloperoxidase. Statistically significant differences between HOMA-IR, TC, TG, and inflammatory markers (TNF-alpha, IL-2) were exhibited by the three genotype models.ConclusionThe subjects and controls in this investigation have significantly different rs7799039 genotype distributions. To acquire a better knowledge of the leptin gene’s role in polycystic ovarian syndrome, more research on a larger population of PCOS patients is required.
关键词：KeywordsLeptin gene polymorphism (rs7799039-g2548a)Metabolic markersOxidative stress markersPolycystic ovarian syndrome