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标题：An adult zebrafish model for adherent-invasive Escherichia coli indicates protection from AIEC infection by probiotic E. coli Nissle
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作者：Dhrubajyoti Nag ; Dustin Farr ; Saumya Raychaudhuri 等
期刊名称：iScience
印刷版ISSN：2589-0042
出版年度：2022
卷号：25
期号：7
页码：1-22
DOI：10.1016/j.isci.2022.104572
语种：English
出版社：Elsevier
摘要：SummaryAdherent-invasiveEscherichia coli(AIEC) is an opportunistic pathogen associated with major inflammatory bowel disease, Crohn disease, and ulcerative colitis. Unfavorable conditions push commensal AIEC to induce gut inflammation, sometimes progressing to inflammation-induced colon cancer. Recently, zebrafish have emerged as a useful model to study human intestinal pathogens. Here, a zebrafish model to study AIEC infection was developed. Bath inoculation with AIEC resulted in colonization and tissue disruption in the zebrafish intestine. Gene expression of pro-inflammatory markers including interleukin-1β (IL-1β), tumor necrosis factor alpha (TNFα), interferon-γ (IFNγ), and S100A-10b (akin to human calprotectin) in the zebrafish intestine was significantly induced by AIEC infection. The probioticE. coliNissle 1917 (EcN) was tested as a therapeutic and prophylactic against AIEC infection and reduced AIEC colonization, tissue damage, and pro-inflammatory responses in zebrafish. Furthermore, EcN diminished the propionic-acid-augmented hyperinfection of AIEC in zebrafish. Thus, this study shows the efficacy of EcN against AIEC in an AIEC-zebrafish model.Graphical abstractDisplay OmittedHighlights•AIEC can colonize, invade, and induce inflammation in the zebrafish gut•ProbioticE. coliNissle can protect zebrafish from AIEC infection•EcN is effective both prophylactically and therapeutically against AIEC-induced IBDPathophysiology; Microbiology