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  • 标题:Label-free cell tracking enables collective motion phenotyping in epithelial monolayers
  • 本地全文:下载
  • 作者:Shuyao Gu ; Rachel M. Lee ; Zackery Benson
  • 期刊名称:iScience
  • 印刷版ISSN:2589-0042
  • 出版年度:2022
  • 卷号:25
  • 期号:7
  • 页码:1-14
  • DOI:10.1016/j.isci.2022.104678
  • 语种:English
  • 出版社:Elsevier
  • 摘要:SummaryCollective cell migration is an umbrella term for a rich variety of cell behaviors, whose distinct character is important for biological function, notably for cancer metastasis. One essential feature of collective behavior is the motion of cells relative to their immediate neighbors. We introduce an AI-based pipeline to segment and track cell nuclei from phase-contrast images. Nuclei segmentation is based on a U-Net convolutional neural network trained on images with nucleus staining. Tracking, based on the Crocker-Grier algorithm, quantifies nuclei movement and allows for robust downstream analysis of collective motion. Because the AI algorithm required no new training data, our approach promises to be applicable to and yield new insights for vast libraries of existing collective motion images. In a systematic analysis of a cell line panel with oncogenic mutations, we find that the collective rearrangement metric, D2min,which reflects non-affine motion, shows promise as an indicator of metastatic potential.Graphical abstractDisplay OmittedHighlights•Versatile AI algorithm identifies individual cell tracks in phase contrast images•Motion of cells relative to nearby neighbors may indicate cancer progressionOptical imaging; Cell biology; Technical aspects of cell biology
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