文章基本信息

标题：Differentiation Of Clarias Batrachus, C. Gariepinus And Heteropneustes Fossilis By Pcr-Sequencing Of Mitochondrial 16s Rrna Gene
本地全文：下载
作者：Mohammad Shamimul Alam ; Hawa Jahan ; Rowshan Ara Begum 等
期刊名称：Journal of the Asiatic Society of Bangladesh, Science
印刷版ISSN：2408-8676
出版年度：2015
卷号：41
期号：1
页码：51-58
DOI：10.3329/jasbs.v41i1.46190
语种：English
出版社：Asiatic Society of Bangladesh
摘要：Heteropneustesfossilis, Clariasbatrachus and C. gariepinus are three major catfishes ofecological and economic importance. Identification of these fish species becomes aproblem when the usual external morphological features of the fish are lost or removed,such as in canned fish. Also, newly hatched fish larva is often difficult to identify. PCRsequencingprovides accurate alternative means of identification of individuals at specieslevel. So, 16S rRNA genes of three locally collected catfishes were sequenced after PCRamplification and compared with the same gene sequences available from othergeographical regions. Multiple sequence alignment of the 16S rRNA gene fragments ofthe catfish species has revealed polymorphic sites which can be used to differentiate thesethree species from one another and will provide valuable insight in choosing appropriaterestriction enzymes for PCR-RFLP based identification in future.
关键词：PCR-sequencing;16S rRNA;Heteropneustes;Clarias;Species identification