文章基本信息

标题：Estudo químico e atividade antibacteriana do extrato hidrometanólico do fungo de solo Thichoderma sp FSF12
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作者：Jorgeffson da Silva Cordeiro ; Eduardo Antnio Abreu Pinheiro ; José Edson de Sousa Siqueira 等
期刊名称：Research, Society and Development
电子版ISSN：2525-3409
出版年度：2022
卷号：11
期号：8
页码：1-8
DOI：10.33448/rsd-v11i8.30852
语种：English
出版社：Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
摘要：Bacterial resistance to antibiotics is a serious public health problem, which can lead to a simple limited infection or even death. The search for new compounds and / or extracts that can help fight these infections must be constantly renewed as a strategy to fight bacterial resistance. The objective of this work was to obtain bioactive substances. Thus, the fungus Trichoderma sp FSF12 was initially grown in cereal medium (rice) and after 28 days of growth the hydromethanolic extract was obtained. The extract was tested using the 96-well plate microdilution method against Gram-positive and Gram-negative bacteria and showed good activity. Then, the hydromethanolic extract had its chemical profile determined by HPLC-DAD, which indicated the presence of substances from different classes of secondary metabolites. The extract was fractionated by column chromatography and led to the isolation of the compounds polyunsaturated fatty acid (1), 5'-inosyl (2), tyrosol (3), harzialactone A (4) and 2-anhydromevalonic (5), which had their structures determined by 1D and 2D Nuclear Magnetic Resonance. The results obtained show the importance of searching for new antibacterial agents.
关键词：Trichoderma;Antimicrobial activity;Endophytic fungi;Substances.