文章基本信息

标题：Influência da terapia hormonal com testosterona sobre a remodelao tecidual prostática em ratos adultos saudáveis: emprego da análise fractal como um método diagnóstico
本地全文：下载
作者：Izabella Tossato Abonizio ; Sarah Raquel César Dourado ; Thainá Cavalleri Sousa 等
期刊名称：Research, Society and Development
电子版ISSN：2525-3409
出版年度：2022
卷号：11
期号：7
页码：1-10
DOI：10.33448/rsd-v11i7.29772
语种：English
出版社：Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
摘要：Testosterone therapy is often indicated for the treatment of several pathological conditions related to aging, including osteoporosis and metabolic syndrome. However, some questions persist about the safety of the use of the therapy, due to the increased susceptibility of non-prostatic disease. The histopathological analyzes are based on subjective methodologies, being important to the validation of quantitative methods, such as fractal analysis, to detect subtle morphological changes and transform the complexity of the structures into quantitative analytical data. Therefore, the study investigated the effects of testosterone therapy on the prostatic microenvironment remodeling and the validation of an effective method for tissue analysis. Wistar rats with 150 days olde were divided into two experimental groups (n = 5): Group T: received subcutaneous injections of testosterone cypionate (5mg/kg body weight) diluted in corn oil every other day for 4 weeks; Group C: received subcutaneous injections of corn oil as vehicle. The animals were euthanized at 180 days old, in a CO2 chamber, followed by decapitation. After euthanasia, the ventral prostate was collected, dissected and histologically processed to perform the fractal, stereological and epithelial height analysis. There was a reduction in the fractal dimension in group T, accompanied by an increase in epithelial height. Stereological analysis revealed a decrease in the stromal compartment and an increase in the epithelial compartment. Likewise, the results demonstrate that the fractal analysis was effective in assessing alterations related to hormonal supplementation with testosterone, evidencing prostatic morphological alterations observed in the other methods.
关键词：Prostate ;Testosterone;Fractals;Pathology.