文章基本信息

标题：Investigation of Leifsonia xyli subsp. xyli in insects
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作者：Airan Miguel dos Santos Panta ; Juliana Lopes Souza ; Paulo Roberto Gagliardi 等
期刊名称：Research, Society and Development
电子版ISSN：2525-3409
出版年度：2022
卷号：11
期号：5
页码：1-9
DOI：10.33448/rsd-v11i5.27469
语种：English
出版社：Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
摘要：Leifsonia xyli subsp. xyli, the causal agent of ratoon stunting, is an endophytic bacteria that colonize sugarcane xylem vessels. It is generally accepted that sugarcane is the only natural host of ratoon stunting. The objective of this work was to investigate the presence of bacteria in insects associated with sugarcane in an experimental area cultivated with four varieties. The plants were artificially inoculated with bacteria, the samples of insects were collected from the sugarcane crop. Specific PCR was used with DNA extracted from different insect parts (head, abdomen, or whole-body). The DNA of the bacterium was not detected in those investigated insects, confirming that the host range of the bacterium is restricted to Saccharum spp.
关键词：Saccharum;DNA;Sugarcane ratoon stunting.