文章基本信息

标题：Biopolímeros na conservao de células de rizobactérias em meios de cultura alternativos
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作者：Manuella Costa Souza ; Celso Afonso Lima ; Dalilla Moreira de Oliveira Moura 等
期刊名称：Research, Society and Development
电子版ISSN：2525-3409
出版年度：2022
卷号：11
期号：4
页码：1-12
DOI：10.33448/rsd-v11i4.27490
语种：English
出版社：Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
摘要：The inoculants are formulated with microorganisms of beneficial action for plants in a simple and accessible composition. They need a quality carrier that guarantees the concentration of active cells, in addition to tolerating variations in temperature, humidity, aeration and storage time without presenting high levels of contaminants, making it necessary to use preservatives. Polymers such as carboxymethylcellulose (CMC) and xanthan gum (XG) have been used for the manufacture of inoculants related to their ability to limit heat transfer, and to their rheological properties, which is the measure of the viscosity and flow strain. Thus, the objective of this work was to evaluate the efficiency of these biopolymers in the preservation of Bradyrhizobium elkanii, Bradyrhizobium diazoefficiens and Azospirillum sp. in alternative culture media with 7, 15, 30 45, 60, 90, 120, 150, 180 and 210 days at 5 °C. The inoculants were evaluated at 210 days, checking the pH and counting CFU mL-1 from the plating using the Spread Plate technique. It was concluded that the use of biopolymers was efficient in the conservation and viability of microorganism cells during the storage period, being the culture medium MS2 that obtained the best results in UFC mL-1, obtaining at 210 days 3.0 x108 CFU mL-1 with use of CMC e 1.4 x108 CFU mL-1 with XG for B. elkanii, 1.3 x107 CFU mL-1 with CMC, and 6.0 x106 CFU mL-1 with XG for B. diazoefficiens, 8.5 x108 CFU mL-1 with XG for Azospirillum sp.
关键词：Inoculant;Microrganism;Preservantive.