文章基本信息

标题：Curva de crescimento de calos de Enterolobium contortisiliquum induzidos in vitro
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作者：Filipe Almendagna Rodrigues ; Vytória Piscitelli Cavalcanti ; Joyce Dória 等
期刊名称：Research, Society and Development
电子版ISSN：2525-3409
出版年度：2022
卷号：11
期号：1
页码：1-5
DOI：10.33448/rsd-v11i1.24550
语种：English
出版社：Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
摘要：The objective of this work was to determine in vitro the growth curve of Enterolobium contortisiliquum calli. Calli from E. contortisiliquum cotyledons cultivated in MS medium, supplemented with 0.5 mg L-1 2,4-D + 2.0 mg L-1 picloram + 0.5 mg L-1 kinetin + 2.0 mg L-1 BAP and repeated twice were used., at intervals of 30 days. After the second subculture, the calli growth curve was established by determining the fresh mass (mg) from the day of inoculation (time 0). The experimental design was completely randomized with 17 treatments, constituted by the evaluation intervals (0, 7, 14, 21, 28, 35, 42, 49, 56, 63, 70, 77, 84, 91, 98, 105 and 112 days), and 24 repetitions. E. contortisiliquum presented five phases of callus development: exponential, linear, deceleration, stationary and decay. The calli growth curve for the species E. contortisiliquum started with the exponential phase and remained in this phase from days 0 to 7. The linear growth period in which the cell division rate decreased and the cell area increased was observed between the days 7 and 14. While the deceleration phase occurred from 14 to 28 days. It can be concluded that the growth curve of E. contortisiliquum calli is a sigmoid with five phases and at the end of the deceleration it is time to carry out the calli subculture, on average at 28 days of in vitro culture.
关键词：Plant tissue culture;Growth regulators;Secondary metabolites.