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标题：In Vitro and in Vivo Antimicrobial Effect of Photodynamic Therapy Using a Highly Pure Chlorin e6 against Staphylococcus aureus Xen29
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作者：Jong-Hwan Park ; Mee-Young Ahn ; Yong-Chul Kim 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2012
卷号：35
期号：4
页码：509-514
DOI：10.1248/bpb.35.509
出版社：The Pharmaceutical Society of Japan
摘要：Photodynamic therapy (PDT) has been recommended as an alternative therapy for various diseases including microbial infection. Recently, we developed a new method for the preparation of highly pure chlorin e₆ (Ce₆), which has been widely used as a second-generation photosensitizer. PDT using Ce₆ was very effective for inhibition of in vitro growth of several bacterial strains. To clarify a possibility for its clinical application, in this study, we examined in vitro and in vivo antimicrobial effects of Ce₆-mediated PDT in mice model of skin infection of Staphylococcus aureus Xen29. Inhibition zone analysis and colony forming unit (CFU) count revealed that Ce₆-mediated PDT inhibited effectively in vitro bacterial growth. In addition, biofilm formation ability of S. aureus Xen29 was decreased by Ce₆-mediated PDT. In vivo experiment, mice receiving Ce₆-mediated PDT exhibited less intensity of bioluminescent signal, showing significant inhibition of bacterial growth. Furthermore, in histopathological examination, marked neutrophilic infiltration and massive bacterial colonies were seen in control mice and mice receiving laser or Ce₆ alone, but not in mice treated with PDT. These results suggest that PDT using Ce₆ extracted by our new method can be clinically useful against bacterial infectious diseases.
关键词：antimicrobial therapy;photodynamic therapy;chlorin e6;Staphylococcus aureus Xen29