文章基本信息

标题：trans-Caffeic Acid Stearyl Ester from Paeonia suffruticosa Inhibits Melanin Synthesis by cAMP-Mediating Down-Regulation of α-Melanocyte-Stimulating Hormone-Stimulated Melanogenesis Signaling Pathway in B16 Cells
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作者：Chia-Hua Liang ; Tzung-Han Chou ; Ya-Ping Tseng 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2012
卷号：35
期号：12
页码：2198-2203
DOI：10.1248/bpb.b12-00619
出版社：The Pharmaceutical Society of Japan
摘要：trans -Caffeic acid stearyl ester (TCASE) from the root cortex of Paeonia suffruticosa A NDREWS is a traditional medicinal herb that has several beneficial properties. However, the inhibitory effect of TCASE on melanogenesis has not been explored. In the cell viability assay, TCASE did not show a cytotoxic effect at a dose of 65 µ M for 48 h in B16, HaCaT and Hs68 cells. TCASE considerably inhibits melanin synthesis, and reduces intracellular cyclic adenosine monophosphate (cAMP) levels, tyrosinase activity and L -3-(3,4-dihydroxyphenyl)-alanine (DOPA) oxidase activity in a concentration-dependent manner in the presence of α-melanocyte-stimulating hormone (α-MSH) in B16 cells, and the inhibition efficiency of TCASE exceeds that of ascorbic acid and arbutin. TCASE reduces melanocortin-1 receptor (MC1R), microphthalmia transcription factor (MITF), tyrosinase, tyrosinase-related protein-2 (TRP-2) and TRP-1 mRNA and protein levels in B16 cells. Based on the findings, TCASE is posited to inhibit melanogenesis signaling while suppressing cAMP levels and, subsequently, MC1R, MITF, tyrosinase, TRP-2 and TRP-1 down-regulation, resulting in the suppression of tyrosinase activity, DOPA oxidase activity and melanin synthesis.
关键词：trans-caffeic acid stearyl ester;Paeonia suffruticosa;melanogenesis;tyrosinase