文章基本信息

标题：Alterations of Plasma Levels of Lysophosphatidic Acid in Response to Fasting of Rats
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作者：Masaki Ino ; Yoshibumi Shimizu ; Tamotsu Tanaka 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2012
卷号：35
期号：11
页码：2059-2063
DOI：10.1248/bpb.b12-00497
出版社：The Pharmaceutical Society of Japan
摘要：The aim of this study was to investigate the effect of fasting on in vivo plasma levels of lysophosphatidic acid (LPA), a physiologically important lysophospholipid mediator. We assayed to measure activities of an LPA-producing enzyme (lysophospholipase D) and LPA-degrading enzyme activities (lysophspholipase A, lipid phosphate phosphatase) in rat plasma or blood, by measuring choline, fatty acid and inorganic phosphate, respectively. Both LPA and its precursor lysophosphatidylcholine (LPC) were quantified by liquid chromatography-tandem mass spectrometry. Fasting of rats for 24 h decreased plasma concentrations of oleoyl-, linoleoyl-, arachidonoyl- and docosahexaenoyl-LPAs, but not palmitoyl- and stearoyl-LPAs, possibly due to decreased levels of corresponding LPCs in the plasma and elevated lipid phosphate phosphatase activity for LPAs in the blood. Our results indicate that the in vivo circulating levels of LPAs in rats are affected by fasting.
关键词：autotaxin;lysophospholipase D;lysophosphatidylcholine;lysophospholipase A;lipid phosphate phosphatase;plasma