文章基本信息

标题：Diosgenin from Dioscorea nipponica Ameliorates Diabetic Neuropathy by Inducing Nerve Growth Factor
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作者：Tong Ho Kang ; Eunjung Moon ; Bin Na Hong 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2011
卷号：34
期号：9
页码：1493-1498
DOI：10.1248/bpb.34.1493
出版社：The Pharmaceutical Society of Japan
摘要：Diabetic neuropathy is characterized by axonal degeneration, demyelination, and atrophy in association with failed axonal regeneration, remyelination, and synaptogenesis. Recent reports suggest that reduced levels of nerve growth factor (NGF) may play a significant role in the pathogenesis of diabetic polyneuropathy. In this study, we investigated the regulation of NGF by steroid diosgenin (DG) in a diabetic neuropathy rodent model. We found that DG, the primary spirostane-type steroid in several Dioscorea species, increased NGF levels in the sciatic nerve of diabetic rats. Additionally, DG increased neurite outgrowth in PC12 cells and enhanced nerve conduction velocities in the diabetic neuropathy mouse model. DG-treated diabetic mice showed reduced disarrangement of the myelin sheath and increased area of myelinated axons by electron microscope studies and exhibited improvement in the damaged axons. Our data further suggest that DG increased the nerve conduction velocity through induction of NGF. Thus, our findings indicate that DG, a major sapogenin obtained from Dioscorea nipponica , reverses functional and ultrastructural changes and induces neural regeneration in a diabetic neuropathy model.
关键词：diabetic neuropathy;diosgenin;nerve conductivity;nerve growth factor;neurite outgrowth