文章基本信息

标题：Influence of organo-mineral supplementation on the production of secondary metabolites in in vitro-germinated Bromelia balansae Mez.
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作者：Maria Juíva Marques de Faria Souza ; Douglas Vieira Thomaz ; Leandro Leal Kloppel 等
期刊名称：Research, Society and Development
电子版ISSN：2525-3409
出版年度：2021
卷号：10
期号：11
页码：1-13
DOI：10.33448/rsd-v10i11.18052
语种：English
出版社：Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
摘要：This work details the investigation of the influence of organo-mineral supplementation on the production of secondary metabolites in in vitro-germinated Bromelia balansae Mez. The seeds were germinated in vitro and transplanted to standard soils to undergo organo-mineral supplementation. The fully-grown plants were subjected to qualitative phytopharmacological prospection; quantitative analysis of total phenols and flavonoids; as well as chemical profiling by mass spectrometry. The in vitro germination of B. balansae was successful, with a germination rate of 94.95 %, thereby equaling to “super seeds” reported in literature. Moreover, the organo-mineral supplementation enhances the production of secondary metabolites in both leaves and roots, what therefore leads to higher yield of total phenols and flavonoids. Furthermore, mass spectrometry analysis confirmed the results from phytopharmacological prospection, and allowed the detection of fragments of secondary metabolites from shikimate/polyketide/mevalonate pathways.
关键词：Phytochemistry;phytochemistry; germination; nutrient; micropropagation; mass spectrometry; natural product;Germination in vitro;Nutrient;Mass spectrometry;Natural product.