文章基本信息

标题：Plaque Formation Assay for Human Parainfluenza Virus Type 1
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作者：Keijo Fukushima ; Tadanobu Takahashi ; Masahiro Takaguchi 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2011
卷号：34
期号：7
页码：996-1000
DOI：10.1248/bpb.34.996
出版社：The Pharmaceutical Society of Japan
摘要：Human parainfluenza virus type 1 (hPIV1) generally does not show visible plaques in common cell lines, including Lewis lung carcinoma-monkey kidney (LLC-MK₂) cells, by plaque formation assays for human parainfluenza virus type 3 (hPIV3) and Sendai virus. In several conditions of the plaque formation assay, complete elimination of serum proteins in the overlay medium was necessary for visualization of hPIV1-induced plaque formation in LLC-MK₂ cells. We developed a plaque formation assay for hPIV1 isolation and titration in LLC-MK₂ cells using an initial overlay medium of bovine serum albumin-free Eagle's minimum essential medium containing agarose and acetylated trypsin for 4—6 d followed by a second overlay staining medium containing agarose and neutral red. The assay allowed both laboratory and clinical hPIV1 strains to form large plaques. The plaque reduction assay was also performed with rabbit anti-hPIV1 antibody as a general evaluation model of viral inhibitors to decrease both the plaque number and size. The results indicate that the plaque formation assay is useful for hPIV1 isolation, titration, evaluation of antiviral reagents and epidemiologic research.
关键词：human parainfluenza virus type 1;plaque assay;isolation;Lewis lung carcinoma-monkey kidney cell;antiviral reagent