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标题：Anticancer Activity of S-Allylmercapto-L-cysteine on Implanted Tumor of Human Gastric Cancer Cell
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作者：Yongkyu Lee ; Hejin Kim ; Jinhwa Lee 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2011
卷号：34
期号：5
页码：677-681
DOI：10.1248/bpb.34.677
出版社：The Pharmaceutical Society of Japan
摘要：Allylmercapto glutathione S -conjugate, S -allylmercapto- L -cysteine (SAMC), which is biotransformed from allyl sulfides and from naturally occurring water-soluble garlic derivatives, has been known to inhibit tumorigenesis. We found that SAMC was able to induce apoptosis in gastric cancer cells in vitro . We report that SAMC inhibited tumor growth rate by 31.36% and 37.78% at doses of 100 and 300 mg/kg, respectively. Apoptosis in the implanted tumor cells was manifested by apoptotic characteristics, including morphological changes of chromatin crescent, cell shrinkage and membrane blebbing. The apoptosis index of 100 mg/kg and 300 mg/kg of SAMC was 20.74±2.50% and 30.61±2.42%, respectively, by terminal deoxy-nucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) staining. The positive rate of B-cell lymphoma 2 (bcl-2) protein expression of control, 100 mg/kg SAMC and 300 mg/kg SAMC was 15.20±1.67%, 10.94±1.57%, and 8.24±1.07%, respectively, by immunohistochemical staining. The positive rate of bax protein expression of control, 100 mg/kg SAMC and 300 mg/kg SAMC was 15.30±1.90%, 23.18±1.81%, and 25.26±3.03%, respectively. We also observed decreases in bcl-2 mRNA and increases in bax mRNA by SAMC in a dose-dependent manner by reverse transcription-polymerase chain reaction (RT-PCR). These results suggest that SAMC may regulate bcl-2 and bax to induce apoptosis in transplanted tumor cells.
关键词：apoptosis;S-allylmercapto- L -cysteine;tumor cell;bax protein;B-cell lymphoma 2 protein