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标题：Augmentation of Gene Expression and Production of Promatrix Metalloproteinase 2 by Propionibacterium acnes-Derived Factors in Hamster Sebocytes and Dermal Fibroblasts: A Possible Mechanism for Acne Scarring
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作者：Takashi Sato ; Hirokazu Kurihara ; Noriko Akimoto 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2011
卷号：34
期号：2
页码：295-299
DOI：10.1248/bpb.34.295
出版社：The Pharmaceutical Society of Japan
摘要：Aberrant extracellular matrix (ECM) remodeling in sebaceous glands and pilosebaceous units in the skin is associated with scar formation under acne conditions. To investigate the involvement of Propionibacterium acnes ( P. acnes ), a Gram-positive anaerobic microbial species, in ECM remodeling in sebaceous glands and pilosebaceous units, we examined the effects of P. acnes culture media, formalin-fixed P. acnes , and peptidoglycan (PGN) from Gram-positive bacteria walls on the production of promatrix metalloproteinase 2 (proMMP-2)/progelatinase A in hamster sebocytes and dermal fibroblasts. When hamster sebocytes (1.8×10⁵ cells) and dermal fibroblasts (1×10⁵ cells) were treated with P. acnes culture media and formalin-fixed P. acnes (corresponding to 1×10⁶ and 1×10⁷ bacterial cells), the production of proMMP-2 was augmented. In addition, PGN (5—50 μg/ml) dose-dependently augmented the production of proMMP-2 in both cells. Furthermore, the PGN (50 μg/ml)-augmented proMMP-2 production was resulted from an increase of its transcript. In contrast, there were no changes in cell proliferative activity in either the P. acnes or PGN-treated sebocytes and dermal fibroblasts, indicating that the augmented proMMP-2 production was not due to an increase in cell numbers. Therefore, these results provide novel evidence that PGN transcriptionally up-regulates the production of proMMP-2 in hamster sebocytes and dermal fibroblasts. Given an increase in the quantity of Gram-positive bacteria, including P. acnes in acne lesions, the aberrant ECM degradation may progress in sebaceous glands and pilosebaceous units, which is associated with acne scar formation.
关键词：sebocyte;Propionibacterium acnes;peptidoglycan;matrix metalloproteinase;scar formation;extracellular matrix remodeling