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标题：The DC2.3 Gene in Caenorhabditis elegans Encodes a Galectin That Recognizes the Galactoseβ1→4Fucose Disaccharide Unit
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作者：Yoko Nemoto-Sasaki ; Shunsuke Takai ; Tomoharu Takeuchi 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2011
卷号：34
期号：10
页码：1635-1639
DOI：10.1248/bpb.34.1635
出版社：The Pharmaceutical Society of Japan
摘要：Galectins comprise a large family of β-galactoside-binding proteins in animals and fungi. We previously isolated cDNAs of 10 galectin and galectin-like genes ( lec-1 to lec-6 and lec-8 to lec-11 ) from Caenorhabditis elegans and characterized the carbohydrate-binding properties of their recombinant proteins. In the present study, we isolated cDNA corresponding to an open reading frame of the DC2.3a gene from C. elegans total RNA; this cDNA encodes another potential galectin. A recombinant DC2.3a protein was expressed in Escherichia coli and used for analysis. The protein displayed hemagglutinating activity against rabbit erythrocytes, bound to an asialofetuin-Sepharose column, and was eluted with lactose. Furthermore, frontal affinity chromatography (FAC) analysis confirmed that DC2.3a recognized oligosaccharides with a non-reducing terminal galactose. According to these results, we designated DC2.3 as lec-12 . The carbohydrate-binding property of the recombinant DC2.3a/LEC-12a was essentially similar to that of LEC-6. Additionally, DC2.3a/LEC-12a and LEC-6 showed higher affinities for the galactoseβ1→4fucose (Galβ1→4Fuc) disaccharide than for N -acetyllactosamine. This suggests that the principal recognition unit is the Galβ1→4Fuc disaccharide as in the case of the C. elegans galectins. However, the recombinant DC2.3a/LEC-12a showed weak affinity for N -glycan E3, which was previously shown to be a preferential endogenous ligand for LEC-6. The DC2.3a/LEC-12a endogenous ligand structures appear to be somewhat different but contain the same galactose-fucose recognition motif.
关键词：galectin;Caenorhabditis elegans;frontal affinity chromatography;carbohydrate-binding property;lectin