文章基本信息

标题：Avaliao inflamatória e imunohistoquímica de materiais reparadores bioceramicos após pulpotomia: estudo em ratos wistar
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作者：Diego Valentim ; Carlos Roberto Emerenciano Bueno ; Ana Maria Veiga Vasques 等
期刊名称：Research, Society and Development
电子版ISSN：2525-3409
出版年度：2021
卷号：10
期号：10
页码：1-14
DOI：10.33448/rsd-v10i10.18480
语种：English
出版社：Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
摘要：Pulpotomy is an alternative of conservative treatment which aims to preserve pulp vitality within root portion, with the aid of biocompatible materials on this remaining living tissue. This study aimed to evaluate the biological response of the pulp remnant to Biodentine and white Angelus MTA bioceramic repair cements compared to calcium hydroxide after pulpotomy. Twenty-four male rats had the coronary pulps of the first and second molars exposed and removed with a sharp curette. The remaining pulp tissue received one of the experimental materials: Biodentine, white Angelus MTA or Ca(OH)2 + distilled water and sealed with glass ionomer. One group was sealed directly with glass ionomer as a negative control group. After 7 and 15 days, the animals were euthanized and the pieces submitted to histological processing to evaluate the inflammatory process with HE staining and immunohistochemistry (Fibronectin and Tenascin), through the attribution of scores from 1 to 4. Formation of hard tissue bridging was observed in HE staining, evaluating presence, continuity and morphology. Data were submitted to the Kruskal Wallis and Dunn test (p showed better morphological aspects compared to glass ionomer (p showed complete hard tissue bridge (p obtained greater marking than the glass ionomer for Fibronectin and Tenascin. Biodentine, white MTA Angelus and calcium hydroxide showed the ability to induce mineralization according to the applied methodology. However, Biodentine showed superior tissue response than glass ionomer and calcium hydroxide.
关键词：Pulpotomy;Inflammation;Materials Testing;Endodontics.