文章基本信息

标题：Inhibitory Effects of Coumarins from the Stem Barks of Fraxinus rhynchophylla on Adipocyte Differentiation in 3T3-L1 Cells
本地全文：下载
作者：Eunjin Shin ; Kyeong-Mi Choi ; Hwan-Soo Yoo 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2010
卷号：33
期号：9
页码：1610-1614
DOI：10.1248/bpb.33.1610
出版社：The Pharmaceutical Society of Japan
摘要：In the course of screening anti-adipogenic activity of natural products employing the preadipocyte cell line, 3T3-L1 as an in vitro assay system, the EtOAc fraction of the stem barks of Fraxinus rhynchophylla D ENCE (Oleaceae) showed significant inhibitory activity on adipocyte differentiation as assessed by measuring fat accumulation using Oil Red O staining. Activity-guided fractionation led to the isolation of six coumarins such as esculetin (1), scopoletin (2), fraxetin (3), fraxidin (4) esculin (5) and fraxin (6). Among the six coumarins isolated, esculetin (1) showed the most potent inhibitory activity on adipocyte differentiation, followed by fraxetin (3). Further studies with interval treatment demonstrated that esculetin (1) exerted inhibitory activity on adipocyte differentiation when treated within 2 d (days 0—2) after differentiation induction. We further investigated the effect of esculetin (1) on peroxisome proliferator activated receptor γ (PPARγ), one of the early adipogenic transcription factors. Esculetin (1) significantly blocked the induction of PPARγ protein expression and inhibited adipocyte differentiation induced by troglitazone, a PPARγ agonist. Taken together, these results suggest that esculetin (1), an active compound from F. rhynchophylla , inhibited early stage of adipogenic differentiation, in part, via inhibition of PPARγ-dependent pathway.
关键词：Fraxinus rhynchophylla;adipocyte differentiation;esculetin;3T3-L1;coumarin