文章基本信息

标题：Musca domestica Larva Lectin Induces Apoptosis in BEL-7402 Cells through a Mitochondria-Mediated Reactive Oxygen Species Way
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作者：Qi Zhao ; Xiaohong Cao ; Bin Zeng 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2010
卷号：33
期号：8
页码：1274-1278
DOI：10.1248/bpb.33.1274
出版社：The Pharmaceutical Society of Japan
摘要：A lectin from Musca domestica larva was purified by affinity chromatography on a glactose-Sepharose 4B column. Musca domestica larva lectin (MLL) inhibited the growth of BEL-7402 cells in a time and concentration-dependent way. The results of Hoechst 33258 staining indicated that MLL induce BEL-7402 cells apoptosis based on the typical apoptotic morphological changes. Subsequently, we found that MLL treatment abrogated glutathione antioxidant system and induced mitochondria to generate reactive oxygen species (ROS) accumulation, resulting in reduction of mitochondrial transmembrane potential. The induction of cell apoptosis was caused by the upregulation of Bax, the downregulation of Bcl-2, the cytochrome c release and the activation of the caspases pathways.
关键词：lectin;Musca domestica larva;antitumor;apoptosis;mitochondrial path