文章基本信息

标题：Protective Effects of Korean Mistletoe Lectin on Radical-Induced Oxidative Stress
本地全文：下载
作者：Boh Kyung Kim ; Mi Jin Choi ; Kun Young Park 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2010
卷号：33
期号：7
页码：1152-1158
DOI：10.1248/bpb.33.1152
出版社：The Pharmaceutical Society of Japan
摘要：The radical scavenging effects and protective activities against oxidative stress of Korean mistletoe ( Viscum album coloratum ) lectin were investigated in vitro and with a cellular system using LLC-PK₁ renal epithelial cells. The Korean mistletoe lectin (KML) showed 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity with an IC₅₀ value of 42.6 μg/ml. It also exerted nitric oxide (NO), superoxide anion (O₂⁻), and hydroxyl radical scavenging activities in concentration-dependent manners. These results suggest that KML is a promising antioxidant by scavenging free radicals. Furthermore, under the LLC-PK₁ cellular model, the cells showed declines in viability and increases in lipid peroxidation through oxidative stress induced by sodium nitroprusside (SNP) and pyrogallol, generators of NO and O₂⁻, respectively. However, KML significantly and dose-dependently inhibited cell cytotoxicity and lipid peroxidation. In addition, 3-morpholinosydnonimnie (SIN-1), a generator of peroxynitrite (ONOO⁻) formed by simultaneously releases of NO and O₂⁻, caused cytotoxicity, lipid peroxidation, and NO overproduction in the LLC-PK₁ cells while KML ameliorated ONOO⁻-induced oxidative damage. Furthermore, overexpressions of cyclooxygenase-2 and inducible NO synthase induced by SIN-1 were observed, but KML down-regulated the expression levels of both genes. KML also reduced SIN-1-induced nuclear factor kappa B expression and the phosphorylation of inhibitor kappa B alpha in LLC-PK₁ cells. These results indicate that KML has protective activities against oxidative damage induced by free radicals.
关键词：Korean mistletoe lectin;LLC-PK1 cell;antioxidative effect;cyclooxygenase-2;inducible nitric oxide synthase;nuclear factor kappa B