文章基本信息

标题：Invariant Vα14 Natural Killer T Cell Activation by Edible Mushroom Acidic Glycosphingolipids
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作者：Hirofumi Nozaki ; Saki Itonori ; Mutsumi Sugita 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2010
卷号：33
期号：4
页码：580-584
DOI：10.1248/bpb.33.580
出版社：The Pharmaceutical Society of Japan
摘要：Invariant natural killer T ( i NKT) cells regulate multi-immune response through Th1/Th2 cytokine release triggered by the recognition of CD1d-restricted glycosphingolipid antigens. Here we report that acidic glycosphingolipids (AGLs) of mushroom ( Hypsizigus marmoreus and Pleurotus eryngii ) presented by murine CD1d-transfected rat basophilic leukocytes induced interleukin-2 (IL-2) release from i NKT hybridoma cells. AGL-1, one of the AGLs, containing mannose at the non-reducing ends, induced CD1d-dependent IL-2 release. Al-though α-galactosylceramide (α-GalCer) presented by CD11c-positive cells induced both interferon-γ (IFN-γ) and IL-4 release, all of AGLs presented by CD11c-positive cells and AGL-1 presented by B cells induced IL-4 release from i NKT hybridoma cells. A single intravenous injection of AGLs into B6 mice induced only a little elevation of IL-4 in serum but repeated intravenous injection of AGLs induced prolonged retention of IL-4 in serum; therefore, these results suggested that edible mushroom AGLs might contribute to the retention of immunohomeostasis through the minimum induction of i NKT cell activation in vivo .
关键词：alpha-galactosylceramide;glycosphingolipid;edible mushroom;natural killer T cell;mouse