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标题：Anti-ulcerogenic Effect and HPLC Analysis of the Caffeoylquinic Acid-Rich Extract from Ligularia stenocephala
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作者：Byung-Il Lee ; Agung Nugroho ; Moch Saiful Bachri 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2010
卷号：33
期号：3
页码：493-497
DOI：10.1248/bpb.33.493
出版社：The Pharmaceutical Society of Japan
摘要：The leaves of three Ligularia species belonging to the family Compositae, Ligularia stenocephala , L. fischeri , and L. fischeri var. spiciformis , were qualitatively and quantitatively analyzed on the caffeoylquinic acids by HPLC and subjected to peroxynitrite-scavenging assay. The IC₅₀ of the MeOH extract of L. stenocephala was 1.62±0.03 μg/ml and the major caffeoylquinic acids of L. stenocephala were 5- O -caffeoylquinic acid, 3,5-di- O -caffeoyl- muco -quinic acid, and 3,5-di- O -caffeoylquinic acid. The compositions of caffeoylquinic acids were different for the three plants. Since percentage of total caffeoylquinic acids of the extract was highest (42.20% of the MeOH extract and 94.52% of the BuOH extract) in L. stenocephala and potent in peroxynitrite-scavenging assay, the extracts of L. stenocephala were chosen to perform in vivo anti-ulcerogenic activity. Treatment of mice with the MeOH- and BuOH extracts decreased the diameter of gastric lesions caused by HCl/ethanol- and indomethacin/bethanechol and decreased the volume of gastric juice, suggesting that caffeoylquinic acids have anti-ulcerogenic activity. These results suggest that the leaves of Ligularia species may help prevent or treat gastric ulcers.
关键词：Ligularia stenocephala;Compositae;caffeoylquinic acid;HPLC;peroxynitrite;ulcerogenic