文章基本信息

标题：Transcriptional Activation of Putative Calmodulin Genes Am-cam-1 and Am-cam-2 from Aquilaria microcarpa, in Response to External Stimuli
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作者：Yoshihide Kenmotsu ; Yoshimi Yamamura ; Shinjiro Ogita 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2010
卷号：33
期号：11
页码：1911-1914
DOI：10.1248/bpb.33.1911
出版社：The Pharmaceutical Society of Japan
摘要：A homology-based cloning strategy yielded two cDNA clones designated Am-cam-1 and Am-cam-2 , presumably encoding calmodulin protein from a callus culture derived from the leaf tissues of Aquilaria microcarpa . An appreciable increase in the transcriptional activity of Am-cam-1 was reproducibly observed by exposure of the cell culture to methyl jasmonate, as analyzed by a reverse-transcription polymerase chain reaction. The expression level of the gene also increased when the cells were treated with yeast extract. The transcription of Am-cam-2 was similarly stimulated by the treatment with methyl jasmonate and yeast extract, however, the intensities of the enhanced expression appeared to be lower as compared with that of Am-cam-1 . In contrast, Ca²⁺-ionophore A23187 did not show inducing activity for the expression of these two calmodulin genes. These results suggest that Am-cam-1 and Am-cam-2 and their products play important roles in signal transduction processes in methyl jasmonate- and yeast extract-treated cells of A. microcarpa , accompanying the change in the transcriptional activities.
关键词：methyl jasmonate;calmodulin;gene expression;agarwood;Aquilaria microcarpa