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标题：Antiasthmatic Activity of Luteolin-7-O-glucoside from Ailanthus altissima through the Downregulation of T Helper 2 Cytokine Expression and Inhibition of Prostaglandin E2 Production in an Ovalbumin-Induced Asthma Model
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作者：Meihua Jin ; Ju Hye Yang ; Eunkyung Lee 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2009
卷号：32
期号：9
页码：1500-1503
DOI：10.1248/bpb.32.1500
出版社：The Pharmaceutical Society of Japan
摘要：Previously, we reported that an ethanol extract of Ailanthus altissima has antiinflammatory activity in an ovalbumin (OVA)-sensitized murine asthmatic model. To determine the biological compounds from this plant, luteolin-7- O -glucoside (L7G) was isolated and its antiasthmatic activity was evaluated in an in vivo murine asthmatic model. L7G (10 to 100 mg/kg, per os ( p.o. )) reduced the amount of eosinophil infiltration in bronchoalveolar lavage (BAL) fluid in a dose-dependent manner. In comparison, dexamethasone (5 mg/kg, p.o. ), which was used as a positive control, also strongly inhibited the number of infiltrating eosinophils. L7G inhibited both the prostaglandin E₂ (PGE₂) and serum immunoglobulin E level in BAL fluid in a dose-dependent manner. In addition, L7G inhibited the transcript profiles of interleukin (IL)-4, IL-5, and IL-13 mRNA expression levels in the murine asthma model, as determined using reverse transcription-polymerase chain reaction (RT-PCR). These results suggest that the antiasthmatic activity of L7G in OVA-induced lung inflammation may occur in part via the downregulation of T helper 2 cytokine transcripts as well as the inhibition of PGE₂ production.
关键词：luteolin-7-glucoside;bronchoalveolar lavage;asthma;prostaglandin E2;T helper 2 cytokine