文章基本信息

标题：Direct Monitoring of Interaction between Escherichia coli Proteins, MinC and Monomeric FtsZ, in Solution
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作者：Takashi Okuno ; Machiko Ogoh ; Hiromitsu Tanina 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2009
卷号：32
期号：8
页码：1473-1475
DOI：10.1248/bpb.32.1473
出版社：The Pharmaceutical Society of Japan
摘要：MinC plays an important role in regulation of the cell division site in Escherichia coli . Previous studies using sedimentation and electron microscopic methods suggested that MinC interacts with the FtsZ polymer and inhibits further FtsZ polymerization. However, it is difficult to clarify details regarding specific molecular interactions by such static analytic methods. In this study, a fluorescence resonance energy transfer (FRET) method was developed to directly observe the interaction between Cy3-labeled MinC and Cy5-labeled FtsZ in solution. FRET analysis indicated that MinC interacts with monomeric rather than polymeric FtsZ in solution. This suggests that interactions between monomeric FtsZ and MinC are important for controlling of FtsZ polymerization by MinC.
关键词：fluorescence resonance energy transfer;cell division;inhibitor;FtsZ;MinC