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标题：Docosahexaenoic Acid Induces Adipose Differentiation-Related Protein through Activation of Retinoid X Receptor in Human Choriocarcinoma BeWo Cells
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作者：Kazushige Suzuki ; Katsuhiko Takahashi ; Tomoko Nishimaki-Mogami 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2009
卷号：32
期号：7
页码：1177-1182
DOI：10.1248/bpb.32.1177
出版社：The Pharmaceutical Society of Japan
摘要：Adipose differentiation-related protein (ADRP) is associated with intracellular lipid droplets that accumulate neutral lipids. Here we report that ADRP expression in a human choriocarcinoma cell line, BeWo, is regulated through activation of retinoid X receptor (RXR) and peroxisome proliferator-activated receptor-γ (PPARγ). Incubation with docosahexaenoic acid (DHA) or oleic acid (OA) induced accumulation of triacylglycerol (TG) and ADRP in BeWo cells. DHA-induced ADRP expression was suppressed by RXR-antagonists, PA452 and HX531. However, oleic acid-induced ADRP expression was not blocked by the RXR-antagonists but by a PPARγ-antagonist. Treatment of the cells with RXR-agonists, HX630 and PA024, increased Adrp transcripts, however, they alone did not change the levels of ADRP protein and TG in BeWo cells. Induction of ADRP protein was observed in the presence of a proteasome inhibitor, suggesting that ADRP is degraded under lipid-poor conditions. These results suggest that expression of ADRP is in part regulated by RXR and PPARγ transcription factors, and DHA induces ADRP by acting as an endogenous agonist of RXR.
关键词：docosahexaenoic acid;retinoid X receptor;lipid droplet;BeWo cell;adipose differentiation-related protein;peroxisome proliferator-activated receptor-γ