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标题：Rapid and Sensitive Detection of Lophophora williamsii by Loop-Mediated Isothermal Amplification
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作者：Yohei Sasaki ; Tsuguto Fujimoto ; Masako Aragane 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2009
卷号：32
期号：5
页码：887-891
DOI：10.1248/bpb.32.887
出版社：The Pharmaceutical Society of Japan
摘要：We have developed a convenient method for the detection of Lophophora williamsii using loop-mediated isothermal amplification (LAMP). We designed six species-specific primers for L. williamsii , including two loop primers. This L. williamsii -specific primer set was used for LAMP of total DNA extracted from L. williamsii and from L. diffusa . Real-time monitoring of LAMP was achieved by measuring turbidity due to the formation of magnesium pyrophosphate. Amplification occurred in samples mixed with total DNA from L. williamsii , but not in those mixed with total DNA from L. diffusa . We could also visually observe L. williamsii by adding fluorescent detection reagent to the reaction tube and exposing it to UV light. This new method amplified L. williamsii selectively and is expected to be applicable to the detection of peyote.
关键词：Lophophora williamsii;Cactaceae;peyote;visual detection;loop-mediated isothermal amplification;trnL intron