文章基本信息

标题：Arteriovenous Differences in NO2− Kinetics in Anesthetized Rabbits
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作者：Takaharu Ishibashi ; Naoki Nishizawa ; Mihoko Nomura 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2009
卷号：32
期号：3
页码：399-404
DOI：10.1248/bpb.32.399
出版社：The Pharmaceutical Society of Japan
摘要：Despite of the importance of plasma NO₂⁻ as an index of endothelial nitric oxide (NO) formation and a substrate for NO production, only a limited kinetic knowledge is available in vivo . To address this issue, we intravenously injected NaNO₂ into anesthetized rabbits and quantified changes in arterial and venous plasma NO₂⁻ levels. Plasma NO₂⁻ levels in arterial blood (956±220 n M ) were slightly, but significantly, higher than those in venous blood (889±214 n M ) under control conditions. Although similar half-lives of the NO₂⁻ were observed in arterial and venous plasma, significant arteriovenous differences were observed in the volume of distribution of the central compartment (0.158±0.007 l/kg in arterial blood and 0.295±0.015 l/kg in venous blood) and the peripheral compartment (0.259±0.035 l/kg in arterial blood and 0.135±0.034 l/kg in venous blood). When NOR1 (authentic NO) was injected, increases in NO₂⁻ levels were greater in arterial plasma, and decreases in blood pressure significantly correlated with arterial NO₂⁻ levels only ( r =0.90, p <0.01). These results indicate that changes in NO₂⁻ are larger and more easily detectable as an index of NO formation in arterial plasma.
关键词：plasma nitrite;kinetic parameter;arteriovenous difference;anesthetized rabbit;authentic nitric oxide