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标题：Globotriaosylceramide-Expressing Burkitt's Lymphoma Cells Are Committed to Early Apoptotic Status by Rhamnose-Binding Lectin from Catfish Eggs
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作者：Tasuku Kawano ; Shigeki Sugawara ; Masahiro Hosono 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2009
卷号：32
期号：3
页码：345-353
DOI：10.1248/bpb.32.345
出版社：The Pharmaceutical Society of Japan
摘要：Silurus asotus (catfish) egg lectin (SAL) has a strong affinity to Gal α-linked carbohydrate chains of not only glycoproteins but also glycosphingolipids such as globotriaosylceramide (Gb3). SAL uniformly bound to surfaces of Gb3-expressing (Gb3⁺) Burkitt's lymphoma cells, while Gb3 molecules were interspersed on the surfaces of Gb3⁺ cells. After a short period of treating Raji and Daudi cells with SAL, each cell size was 10 and 25% smaller than that of untreated cells, respectively. Treatment of Gb3⁺ cells with SAL caused an increase in binding of annexin V, however, neither caspase activation nor DNA fragmentation was observed after treatment with SAL for 22 h. Since SAL did not induce cell death in Gb3⁺ cells, SAL may function as an inducer of early apoptotic signal. We have revealed that SAL did not bind to D - threo -1-phenyl-2-decanoylamino-3-morphorino-1-propanol ( D -PDMP)-treated Raji cells, and no cell shrinkage was observed in Gb3-deficient Raji cells treated with SAL, indicating that Gb3 localized in the glycosphingolipid-enriched microdomain (GEM) was involved in SAL-induced cell shrinkage through activation of voltage-gated potassium channel Kv1.3, and that the glycoprotein ligands on Gb3-deficient Raji cells treated with SAL were not included in this phenomenon. These results suggest that SAL leads the cells to early apoptotic status via binding to Gb3 existing in GEM, and that this binding is a prerequisite condition to induce early stage of apoptosis.
关键词：Burkitt's lymphoma cell;apoptosis;cell size;rhamnose-binding lectin;fish egg;glycosphingolipid-enriched microdomain