文章基本信息

标题：Some Properties of β-1,2-Mannosyltransferases Related to the Biosynthesis of the Acid-Labile Oligomannosyl Side Chains in Candida albicans NIH B-792 Strain Cells
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作者：Kouji Goto ; Akifumi Suzuki ; Nobuyuki Shibata 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2009
卷号：32
期号：11
页码：1921-1923
DOI：10.1248/bpb.32.1921
出版社：The Pharmaceutical Society of Japan
摘要：We detected the β-1,2-mannosyltransferases (β-1,2-MTs), which participate in the biosynthesis of oligomannosyl side chains in the mannan acid-labile fraction, in a particulate insoluble fractions prepared from Candida albicans NIH B-792 strain cells grown at 27 °C and at 37 °C in a yeast extract-added Sabouraud liquid medium (YSLM). The β-1,2-MT VI-6 prepared from the cells grown at 27 °C exhibited the maximum activity at pH 7.0 and at 30 °C. The β-1,2-MT VI-6 activity was only slightly affected by Mn²⁺, Mg²⁺, Ca²⁺, and ethylenediaminetetraacetic acid, but completely inhibited by Zn²⁺ and Ni²⁺. The β-1,2-MT activities from the cells grown at 37 °C were lower than that from the cells grown at 27 °C, especially on the longer β-1,2-mannooligosaccharides than tetraose.
关键词：Candida albicans;mannosyltransferase;β-1,2-mannooligosaccharide;temperature