文章基本信息

标题：Differential Effect of Resveratrol on Nitric Oxide Production in Endothelial F-2 Cells
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作者：Satoru Takahashi ; Tomomi Uchiyama ; Ken-ichi Toda 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2009
卷号：32
期号：11
页码：1840-1843
DOI：10.1248/bpb.32.1840
出版社：The Pharmaceutical Society of Japan
摘要：We examined the rapid effect of resveratrol on nitric oxide (NO) production in endothelial F-2 cells. During an incubation period of 10 min, resveratrol at low concentrations (<20 μ M ) had no effect on NO production, whereas it significantly increased NO production at high concentrations (>50 μ M ). In contrast, pretreatment with resveratrol at low concentrations caused a significant decrease in vascular endothelial growth factor (VEGF)-stimulated NO production. Resveratrol failed to induce phosphorylation of endothelial NO synthase (eNOS) and VEGF receptor and did not affect autophosphorylation of the VEGF receptor. However, resveratrol markedly suppressed VEGF-induced eNOS phosphorylation. Resveratrol at high concentrations reduced the viability of F-2 cells as determined by 3-(4,5-dimethyl-2-thiazoyl)-2,5-diphenyl-2 H -tetrazolium bromide (MTT) and dye exclusion methods. Resveratrol-stimulated NO production was completely abolished by the depletion of extracellular Ca²⁺. These results indicate that resveratrol stimulates NO production by a Ca²⁺-dependent mechanism and reduces VEGF-stimulated NO production by impairing a Ca²⁺-independent mechanism in endothelial F-2 cells. However, the stimulatory effect of resveratrol may be partly attributed to its cytotoxicity.
关键词：resveratrol;endothelial cell;nitric oxide